Synthesis of glycopolymers with specificity for bacterial strains via bacteria-guided polymerization

Yan Luo ab, Yan Gu ab, Ruyan Feng ab, John Brash ac, Ahmed M. Eissa d, David M. Haddleton*d, Gaojian Chen *ab （陈高健）and Hong Chen *a（陈红）

a The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren-Ai Road, Suzhou, 215123, P. R. China.

b Center for Soft Condensed Matter Physics and Interdisciplinary Research School of Physical Science and Technology, Soochow University, Suzhou, 215006, P. R. China.

c School of Biomedical Engineering and Department of Chemical Engineering, McMaster University, Hamilton, Ontario, L8S4L7, Canada.

d Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.

Chem. Sci., 2019, 10, 5251--5257

Identifying probiotics and pathogens is of great interest to the health of the human body. It is critical to develop microbiota-targeted therapies to have high specificity including strain specificity. In this study, we have utilizedE. coliMG1655 bacteria as living templates to synthesize glycopolymersin situwith high selectivity. By this bacteria-sugar monomer-aptation-polymerization (BS-MAP) method, we have obtained glycopolymers from the surface of bacteria which can recognize template bacteria from two strains ofE. coliand the specific bacteria-binding ability of glycopolymers was confirmed by both bacterial aggregation experiment and QCM-D measurements. Furthermore, the synthesized glycopolymers have shown a powerful inhibitory ability which can prevent bacteria from harming cells in both anti-infection and co-culture tests.

链接：https://pubs.rsc.org/en/content/articlelanding/2019/SC/C8SC05561K#!divAbstract